Ilham Ben Yelles
University of Oran, Algeria
Title: Antimicrobial and antioxidant activity of essential oil of cumin dental caries on Streptococcus sanguinis involved on dental caries diabetes mellitus
Biography
Biography: Ilham Ben Yelles
Abstract
Introduction: Diabetes mellitus is one of the most common chronic diseases in children. However, several studies have shown that unbalanced diabetes is often associated with changes in the oral environment namely altered buffering capacity, xerostomia, high numbers of Streptococci and Lactobacilli which increases the risk of dental caries.
Aim: To reduce the appearance of Streptococcus sanguinis involved in dental caries.
Materials & Methods: After recruiting diabetic children, two young patients responding to informed consent, underwent salivary sampling for isolation and identification of the bacterium, microbial activity of the extracted oil was evaluated by the disc diffusion method, then a minimal inhibitory concentration was sought, then antioxidant activity was achieved by the free radical trapping method DPPH
Results: Carious disease is present in 80% of diabetic children, 72% had unbalanced diabetes with an average HbA1C level of 8.1+/-1.6%. The disc diffusion method has shown that essential oil of cumin has intermediate activity on Streptococci sanguinis. The inhibition diameter is 14mm. This oil also has a more interesting antioxidant activity (CI50=0.02) than ascorbic acid (CI50=0.048) All experiments were performed in triplicate. Data were analyzed using SPSS software 21.0 (Chicago, IL, USA). The data are expressed as the mean±standard deviation values. The statistical analysis was done using Student's t-test. Values of p<0.05 were considered statistically significant.
Conclusion: Given the high prevalence of carious disease in diabetic children. The essential oil of cumin revealed an interesting intermediate activity on S. sanguinis, to prevent the installation of the lesion.